Spectroscopic study of protein secondary structure in biomaterials for focal treatments

I investigated efficacy and dosage of focal therapies for tumor ablation in the treatment of cancer and pulmonary vein ablation for atrial fibrilation. I worked with animal models, in vitro models and numerical models of these systems. I performed experiments with modes of focal therapy such as heat, cryo and irreversible electroporation. I correlated the viability and thermal damage to the change in protein structure of cells and tissues using Fourier Transform Infrared (FTIR) spectroscopy and Differential scanning calorimetry (DSC), fitted parameters to a thermal damage model and performed heat transfer simulations to predict ablation region in vivo using the commercial package COMSOL.

I communicated updates regularly on my progress and collaborated with a large and diverse team of researchers from biology, medical and engineering fields. I picked up new skills such as cell culture, viability assays and research animal handling to complement my technical skillset. Out of the three projects described below, I led one project and played critical roles in the other two.